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caspase 1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc caspase 1
    Caspase 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 2134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+1+2225/Caspase-1+Antibody/pm41877597-142-65-67
    Average 97 stars, based on 2134 article reviews
    caspase 1 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Infection:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Imaging:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Expressing:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Membrane:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Western Blot:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Microscopy:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Concentration Assay:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Activity Assay:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Virus:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).

    Control:

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV-2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/DIABLO (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories (San Jose, CA, USA).

    Article Title: SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia
    Article Snippet: The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).The proteins were electrotransferred to a polyvinylidene difluoride (PVDF) membrane (Life Technologies, Carlsbad, CA, USA) and detected using the indicated antibodies and ECL-Plus Western Blotting Detection System (GE Healthcare, Piscataway, NJ, USA).. Antibodies against cleaved caspase 7 (9491), caspase 7 (12827), SARS-CoV2 N-protein (33717), AIF (5318), p-Drp1 (ser616) (3455), Smac/Diablo (15108), Caspase 1(2225) and Bid (2002) were purchased from Cell Signaling Technology (Beverly, MA, USA).. Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).Total Drp1antibody (611112) was obtained from BD Transduction Laboratories, San Jose, CA, USA).



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    Schematic illustration of the mechanism of ZIF-8@Que nanoparticles in GC treatment. ZIF-8@Que nanoparticles were prepared via a one-pot co-precipitation process, encapsulating quercetin within the ZIF-8 framework. Upon systemic administration, ZIF-8@Que preferentially accumulates in gastric tumors and undergoes acidic pH-triggered drug release. The nanoparticles exhibit intrinsic POD-like activity, catalyzing H 2 O 2 to generate ROS, which disrupt mitochondrial function by collapsing Δψm, depleting ATP, and enhancing oxidative stress. ROS-driven mitochondrial injury subsequently activates inflammasome-dependent caspase-1, leading to GSDMD cleavage, pore formation, release of IL-1β and IL-18, and subsequent pyroptotic cell death. This cascade culminates in robust eradication of GC cells while sparing normal tissues, highlighting the therapeutic promise of ZIF-8@Que as pyroptosis-inducing nanoparticles.

    Journal: Materials Today Bio

    Article Title: pH-responsive ZIF-8@quercetin nanoparticles induce pyroptosis for targeted gastric cancer therapy

    doi: 10.1016/j.mtbio.2026.102806

    Figure Lengend Snippet: Schematic illustration of the mechanism of ZIF-8@Que nanoparticles in GC treatment. ZIF-8@Que nanoparticles were prepared via a one-pot co-precipitation process, encapsulating quercetin within the ZIF-8 framework. Upon systemic administration, ZIF-8@Que preferentially accumulates in gastric tumors and undergoes acidic pH-triggered drug release. The nanoparticles exhibit intrinsic POD-like activity, catalyzing H 2 O 2 to generate ROS, which disrupt mitochondrial function by collapsing Δψm, depleting ATP, and enhancing oxidative stress. ROS-driven mitochondrial injury subsequently activates inflammasome-dependent caspase-1, leading to GSDMD cleavage, pore formation, release of IL-1β and IL-18, and subsequent pyroptotic cell death. This cascade culminates in robust eradication of GC cells while sparing normal tissues, highlighting the therapeutic promise of ZIF-8@Que as pyroptosis-inducing nanoparticles.

    Article Snippet: Membranes were blocked with 5 % milk and incubated overnight at 4 °C with primary antibodies against: NDUFS1 (1:10000, Abcam), SDHA (1:1000, CST), Drp1 (1:1000, Abcam), p-Drp1 (1:1000, Abcam), Mfn2 (1:1000, Abcam), Caspase-3 (1:5000, Abcam), Cleaved Caspase-3 (1:500, Abcam), Bcl-2 (1:2000, Abcam), Bax (1:1000, Abcam), Caspase-1 (1:1000, Abcam), Cleaved Caspase-1 (1:1000, CST), GSDMD-FL (1:1000, CST), Cleaved GSDMD (1:1000, CST), GSDME (1:1000, CST), Cleaved GSDME (1:1000, CST), GPX4 (1:1000, CST), ACSL4 (1:1000, CST) and β-Actin (1:1000, CST).

    Techniques: Activity Assay

    In vivo therapeutic efficacy and safety profile of ZIF-8@Que. (A) Hemolysis assay of mouse RBCs incubated with ZIF-8@Que at various concentrations. (B, C) Assessment of systemic toxicity in healthy mice: body weight (B) and serum biochemical (C) of C57BL/6 mice receiving repeated intravenous doses of ZIF-8@Que (50 mg/kg, once weekly for 4 weeks). (D) H&E staining of major organs confirming normal histology. Scale bar = 10 μm. (E) Experimental timeline for tumor establishment and treatment in nude mice. (F) Body weight of tumor-bearing mice during therapy. (G, H) Tumor growth curves (G) and tumor weight curves (H) for each group at different time points. (I) Kaplan-Meier survival curves for each group. (J) Representative tumor images at study endpoint. (K) H&E staining showing extensive necrosis in ZIF-8@Que-treated tumors. (L) Representative images of Ki67, cleaved caspase-1, cleaved caspase-3, cleaved GSDMD and TUNEL in tumor tissues, highlighting pyroptosis-mediated antitumor effects. For all studies, n ≥ 3. Data are shown as the mean ± SD. Comparisons were performed using the student's t-test or ANOVA. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, or ns = not significant.

    Journal: Materials Today Bio

    Article Title: pH-responsive ZIF-8@quercetin nanoparticles induce pyroptosis for targeted gastric cancer therapy

    doi: 10.1016/j.mtbio.2026.102806

    Figure Lengend Snippet: In vivo therapeutic efficacy and safety profile of ZIF-8@Que. (A) Hemolysis assay of mouse RBCs incubated with ZIF-8@Que at various concentrations. (B, C) Assessment of systemic toxicity in healthy mice: body weight (B) and serum biochemical (C) of C57BL/6 mice receiving repeated intravenous doses of ZIF-8@Que (50 mg/kg, once weekly for 4 weeks). (D) H&E staining of major organs confirming normal histology. Scale bar = 10 μm. (E) Experimental timeline for tumor establishment and treatment in nude mice. (F) Body weight of tumor-bearing mice during therapy. (G, H) Tumor growth curves (G) and tumor weight curves (H) for each group at different time points. (I) Kaplan-Meier survival curves for each group. (J) Representative tumor images at study endpoint. (K) H&E staining showing extensive necrosis in ZIF-8@Que-treated tumors. (L) Representative images of Ki67, cleaved caspase-1, cleaved caspase-3, cleaved GSDMD and TUNEL in tumor tissues, highlighting pyroptosis-mediated antitumor effects. For all studies, n ≥ 3. Data are shown as the mean ± SD. Comparisons were performed using the student's t-test or ANOVA. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, or ns = not significant.

    Article Snippet: Membranes were blocked with 5 % milk and incubated overnight at 4 °C with primary antibodies against: NDUFS1 (1:10000, Abcam), SDHA (1:1000, CST), Drp1 (1:1000, Abcam), p-Drp1 (1:1000, Abcam), Mfn2 (1:1000, Abcam), Caspase-3 (1:5000, Abcam), Cleaved Caspase-3 (1:500, Abcam), Bcl-2 (1:2000, Abcam), Bax (1:1000, Abcam), Caspase-1 (1:1000, Abcam), Cleaved Caspase-1 (1:1000, CST), GSDMD-FL (1:1000, CST), Cleaved GSDMD (1:1000, CST), GSDME (1:1000, CST), Cleaved GSDME (1:1000, CST), GPX4 (1:1000, CST), ACSL4 (1:1000, CST) and β-Actin (1:1000, CST).

    Techniques: In Vivo, Drug discovery, Hemolysis Assay, Incubation, Staining, TUNEL Assay